补阳还五汤对局灶性脑缺血大鼠脑内caveolin的影响

Objective:To investigate the effect of BYHW decoction on expression of caveolin in a rat model of cerebral ischemia. Methods: Brain ischemia was induced by middle cerebral artery occlusion. The rats were randomized into four groups including naive, shame-operated, ischemia, and ischemia treated with BuYangHuanWu Decoction (BYHW). The BYHW groups of rats were then orally administered daily with the BYHW and killed at 12h,1, 3 or 7day point after operation. Caveolin1 and caveolin2 were measured by immunohistochemical staining. Results: There were some Caveolin 1 and 2 positive cells in normal brain , expression of caveolin 1 or 2 was enhanced immediately after ischemia and reached peak at 3 day or 1 day after ischemia respectively ; BYHW decoction increased the number of caveolin1-positive or caveolin2-positive cells. This increase was statistically significant at 3 and 7 day after ischemia and treatment. Conclusion: BYHW decoction is capable of regulating expression of caveolin 1 and 2 in ischemic brain , that may be an anti-ischemic mechanism of BYHW decoction.