杨树全基因组NBS类型抗病基因分析
Genome-Wide Analysis of NBS-Encoding Disease Resistance Genes in Populus trichocarpa
本研究对杨树（Populus trichocarpa）中含有核苷酸结合区（NBS）结构的候选抗病基因进行了全基因组的分析研究，包括NBS类型基因总量的的确认、类型分析、物理位置分布和系统发生学关系分析等。研究结果表明，在杨树全基因组中找到381个有NBS结构的基因，按照N端结构和亮氨酸富集区（LRRs）结构的区别分为122个非标准结构NBS基因、259个标准结构NBS基因，其中标准类基因又分为13个小类,主要包括TNL、CNL、NL、XNL、TN五大类型和其他一些小类。同时，研究发现81.9%的NBS基因分布在一定的簇内，说明杨树基因组中抗病基因在进化过程中存在更多的复制现象。通过对基因组内标准NBS基因进行系统进化树分析，发现杨树抗病基因存在明显的多分枝，这与水稻和拟南芥存在显著差异。
In research paper, a full set of disease resistance (R) candidate genes encoding nucleotide-binding sites (NBS) in a complete genome of Populus trichocarpa was identified and characterized by structural diversities, physical positions, phylogenetic relationships. Characterizing by N-terminal motif and leucine-rich repeat domains motif, We found 381 NBS-coding sequences which contain 122 non-regular NBS genes and 259 regular NBS genes that classified 13 types such as TNL, CNL、NL、XNL、TN and other types. .Meanwhile 81.9% of the NBS genes were located in gene clusters showed that there are more copy phenomenon in the evolution of disease resistance genes of Populus trichocarpa. After analysis of NBS standard phylogenetic tree in the genome of Populus trichocarpa, many significantly branches were found. It was significantly different with rice and Arabidopsis.
项艳、蔡诚、吴大强、魏国
遗传学分子生物学植物学
杨树生物信息学抗病基因NBS系统进化树
Populus trichocarpabioinformaticsdisease resistance geneNucleotide binding sitePhylogenetic tree
项艳,蔡诚,吴大强,魏国.杨树全基因组NBS类型抗病基因分析[EB/OL].(2007-10-29)[2025-08-21].http://www.paper.edu.cn/releasepaper/content/200710-514.点此复制
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