染色葡萄花色苷合成关键转录因子VvMYBA1启动子的序列克隆与功能分析

nthocyanins are important water soluble flavonoid pigments in winemaking red grapes, which were only accumulated in the skins of the matured red grapes normally. But for the "teinturier" grapes, anthocyanins could accumulate greatly in both of their skins and pulps. The present study was aim to investigate the potential functions and effects of the key transcription factor VvMYBA1 in the anthocyanin tissue-specific biosynthesis of its pulps. Therefore, its DNA sequence was cloned by using molecular biology technology, its potential functions was predicted with the help of bioinformatics, and its basic functions were tested by using infecting of promoter combinant vector on tobacco leaves. This research revealed that the promoteSequence Cloning and Functional Analysis for the Promoter of the Key Transcription Factor VvMYBA1 in Anthocyanin Biosynthesis in the Teinturier grape Vitis Vinifera L. cv. 'Tintorera'r sequence of VvMYBA1 of the teinturier grape cultivar 'Tintorera' was the same with the reported one from 'Pinot Noir', which had various photoresponse elements, as well as the response sites to other different regulators or phytohormone signals. Besides, the function test revealed that the VvMYBA1 promoter had the basic biological functions of promoters, the combinant vector of which could start the great expression of the report gene on tobacco leaves．