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中式辣椒酱生产过程中细菌群落组成及动态变化

omposition and dynamic changes of bacterial communities in the manufacture process of Chinese chili sauce

中文摘要英文摘要

以辣椒酱生产中的三工段样本为原料,运用16S rRNA扩增子测序,对腌渍辣椒、炒制辣椒酱和成品辣椒酱中的菌群结构及其动态变化进行分析,并将其与辣椒酱中的环境因子加以联系。结果表明辣椒酱生产过程中菌群结构变化显著。嗜酸乳杆菌、戊糖乳杆菌作为优势菌贯穿整个生产工段。腌渍辣椒中的优势菌为发酵乳杆菌,炒制与成品辣椒酱中的优势菌为敏捷乳杆菌。环境因子中盐度、总酸和可溶性糖含量对菌群结构有显著影响。

nalysis of the bacterial communities of chili sauce in the production process was of vital importance to monitor the condition of chili sauce. This study aimed to determine how the structure of bacterial communities changed throughout the chili sauce production process and their relationship with environmental factors.The composition and relative abundances of bacteria in the three stages of chili sauce production, including pickled chili, fried chili and finished product, were investigated using 16S rRNA gene (S) amplicon sequencing and the influence of environmental factors were also investigated. The results showed that the bacterial diversity in the chili sauce production process was high. Lactobacillus acidipiscis and L. pentosus were dominant throughout the entire production process of chili sauce. Large amount of L. fermentum was found in salted chili while L. agilis was present at high abundances in the pickled chili and final product, respectively. As for environmental factors, salt, total acid and soluble sugar content were found to influence the bacterial communities significantly. A high level of bacterial diversity existed in the chili sauce and the dominant bacterial species in the chili sauce production process varied across different production stages.

钮成拓、刘春凤、王琪、郑飞云、李崎、李永仙、王金晶、徐廷弼

微生物学生物科学研究方法、生物科学研究技术农艺学

发酵工程16S rRNA扩增子测序细菌群落辣椒酱动态变化环境因子

Fermentation engineering16S rRNA gene (S) amplicon sequencingBacterial communityChili sauceDynamic changesEnvironmental factors.

钮成拓,刘春凤,王琪,郑飞云,李崎,李永仙,王金晶,徐廷弼.中式辣椒酱生产过程中细菌群落组成及动态变化[EB/OL].(2018-03-28)[2025-08-03].http://www.paper.edu.cn/releasepaper/content/201803-252.点此复制

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