Alternate primers for whole-genome SARS-CoV-2 sequencing
Alternate primers for whole-genome SARS-CoV-2 sequencing
Abstract As the world is struggling to control the novel Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), there is an urgency to develop effective control measures. Essential information is encoded in the virus genome sequence with accurate and complete SARS-CoV-2 sequences essential for tracking the movement and evolution of the virus and for guiding efforts to develop vaccines and antiviral drugs. While there is unprecedented SARS-CoV-2 sequencing efforts globally, approximately 19 to 43% of the genomes generated monthly are gapped, reducing their information content. The current study documents the genome gap frequencies and their positions in the currently available data and provides an alternative primer set and a sequencing scheme to help improve the quality and coverage of the genomes.
Kaleebu Pontiano、Bugembe Dan Lule、Phan My V.T.、Cotten Matthew
UK Medical Research Council¨CUganda Virus Research Institute and London School of Hygiene and Tropical Medicine Uganda Research Unit||Uganda Virus Research InstituteUK Medical Research Council¨CUganda Virus Research Institute and London School of Hygiene and Tropical Medicine Uganda Research UnitErasmus Medical CenterUK Medical Research Council¨CUganda Virus Research Institute and London School of Hygiene and Tropical Medicine Uganda Research Unit||UK Medical Research Council¨CUniversity of Glasgow Centre for Virus Research
医学研究方法分子生物学微生物学
SARS-CoV-2COVID-19 primersnext generation sequencing
Kaleebu Pontiano,Bugembe Dan Lule,Phan My V.T.,Cotten Matthew.Alternate primers for whole-genome SARS-CoV-2 sequencing[EB/OL].(2025-03-28)[2025-05-22].https://www.biorxiv.org/content/10.1101/2020.10.12.335513.点此复制
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