鱼藤根中鱼藤素的提取分离及HPLC分析

hree methods were applied to extract deguelin from Derris trifoliate roots. Different solvents were used to separate rotenoids, such as deguelin, rotenone and elliptone. The weight of total extract and the relative deguelin extraction efficiency were determined. The total amount of the extracted substance assisted by using ultrasound was lower than the Sohxlet or vibrating extraction method. However, deguelin extraction rate by using the ultrasound-assisted extraction was the highest. Furthermore, with respect of rotenoids separation, the solvent mixture of petroleum ether:chloroform:acetone at the ratio of 6:1:1 was found to be most effective. The maximum optical absorption of deguelin occurred at 271.6 nm. It suggested that the deguelin and rotenone content could be determined at 280 nm.