III过氧化氢酶和过氧化物酶活性观察

Objective To observe whether CAIII in skeletal muscle has catalase or peroxidase activity. Methods After extracting from skeletal muscle, the proteins were separated by means of SDS-PAGE. Kaumas blue staining and western blot staining were used to determine the location of CAIII on the gel. Phosphatase staining was used to identify if CAIII still has enzyme activity. Iron staining and DAB staining were used to observe whether CAIII has catalase or peroxidase activity.Results CAIII in skeletal muscle could react immunologically with anti-CAIII polyclonal antibody. According to its location, CAIII could be identified from complex protein components on the Kaumas blue staining map. Phosphatase staining showed that CAIII still has enzyme activity. However, we did not find the corresponding CAIII strip by iron staining and DAB staining.Conclusion CAIII may not have catalase activity, and is also not a peroxidase isozyme. The mechanism of its resistence to oxidative damage need to further study.