In vitro broad-spectrum antiviral activity of MIT-001, a mitochondria-targeted reactive oxygen species scavenger, against severe acute respiratory syndrome coronavirus 2 and multiple zoonotic viruses
In vitro broad-spectrum antiviral activity of MIT-001, a mitochondria-targeted reactive oxygen species scavenger, against severe acute respiratory syndrome coronavirus 2 and multiple zoonotic viruses
Abstract The COVID-19 pandemic caused by SARS-CoV-2 becomes a serious threat to global health and requires the development of effective antiviral therapies. Current therapies that target viral proteins have limited efficacy with side effects. In this study, we investigated the antiviral activity of MIT-001, a small molecule reactive oxygen species (ROS) scavenger targeting mitochondria, against SARS-CoV-2 and other zoonotic viruses in vitro. The antiviral activity of MIT-001 was quantified by RT-qPCR and plaque assay. We also evaluated the functional analysis of MIT-001 by JC-1 staining to measure mitochondrial depolarization, total RNA sequencing to investigate gene expression changes, and immunoblot to quantify protein expression levels. The results showed that MIT-001 effectively inhibited the replication of B.1.617.2 and BA.1 strains, Zika virus, Seoul virus, and Vaccinia virus. Treatment with MIT-001 restored the expression of heme oxygenase-1 (HMOX1) and NAD(P)H: quinone oxidoreductase 1 (NqO1) genes, anti-oxidant enzymes reduced by SARS-CoV-2, to normal levels. The presence of MIT-001 also alleviated mitochondrial depolarization caused by SARS-CoV-2 infection. These findings highlight the potential of MIT-001 as a broad-spectrum antiviral compound that targets for zoonotic RNA and DNA viruses, providing a promising therapeutic approach to combat viral infection.
Kim Won-Keun、Kim Soon Ha、Rajoriya Shivani、Yoo Junsang、Kim Jong Woo、Im Hyeonjoo、Lee Eun-Woo、Shim Hyun Soo、Natasha Augustine、Shin Hye Jin、Lim Taehun、Kim Bohyeon
Department of Microbiology, College of Medicine, Hallym University||Institute of Medical Science, College of Medicine, Hallym UniversityMitoimmune Therapeutics IncDepartment of Microbiology, College of Medicine, Hallym UniversityLaboratory of regenerative medicine for neurodegenerative diseaseMetabolic Regulation Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)||Department of Functional Genomics, University of Science and Technology (UST)Laboratory of regenerative medicine for neurodegenerative diseaseMetabolic Regulation Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)||Department of Functional Genomics, University of Science and Technology (UST)||School of pharmacy, Sungkyunkwan UniversityLaboratory of regenerative medicine for neurodegenerative diseaseDepartment of Microbiology, College of Medicine, Hallym UniversityCollege of Medicine, Chungnam National UniversityDepartment of Microbiology, College of Medicine, Hallym UniversityDepartment of Microbiology, College of Medicine, Hallym University
医学研究方法基础医学生物科学研究方法、生物科学研究技术
COVID-19MIT-001ROSHMOX1NqO1Broad-spectrum antiviral
Kim Won-Keun,Kim Soon Ha,Rajoriya Shivani,Yoo Junsang,Kim Jong Woo,Im Hyeonjoo,Lee Eun-Woo,Shim Hyun Soo,Natasha Augustine,Shin Hye Jin,Lim Taehun,Kim Bohyeon.In vitro broad-spectrum antiviral activity of MIT-001, a mitochondria-targeted reactive oxygen species scavenger, against severe acute respiratory syndrome coronavirus 2 and multiple zoonotic viruses[EB/OL].(2025-03-28)[2025-05-15].https://www.biorxiv.org/content/10.1101/2023.07.06.547945.点此复制
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