雷公藤细胞固定化细胞的生长特征及甲素合成

In this study, Tripterygium wilfordii cells embedded in Sodium alginate was used to construct the system of immobilized cell culture for study the relationship between cell culture and tirpolide compound. The results showed that there existed 3 growth stages in cell culture, namely slow growth stage, exponential growth stage, and steady growth stage. Meanwhile, the contents of total sugar, rapeseed meal protein, MDA decreased as culture time increased. However, PH value first increased and then decreased, and cell viability first decrease and then increased. In addition, in the process of culture, the change of POD showed a curve of "V" with the lowest value in sixth day, the change of SOD showed a smooth curve, and the change of PAL enzyme showed a trend that it first increased sharply and then decreased slowly. While the content of triptolide increased with the culture time and reach a maximum yield of 66.19 μ g/g and 4.91 mg/L inside and outside the cells.