体外暴露全氟辛烷磺酸（PFOS）对淋巴细胞增值功能的影响

Objective To assess the effects of lymphocyte proliferation function by perfluorooctane sulfonate (PFOS) exposure in vitro which provide scientific evidence for further revealing immunotoxicity mechanism of PFOS. Methods Splenocytes from C57BL/6 mice were treated with PFOS at different concentration in vitro (0, 1, 5, 10, 50,200, 300μM), and then after 48 hours, the proliferation ability of the T and B lymphocytes was determined by MTT stain assay with mitogen concanavalin (Con) A or lipopolysaccharide (LPS) stimulating, respectively. Results The results showed that the T lymphocytes proliferation function was decreased significantly in 300μM PFOS group comparison with to control group, and the B lymphocytes proliferation function was decreased significantly began in 200μM PFOS group comparison with to control group. Conclusion This study indicates that PFOS exposure in vitro may decreases the lymphocyte proliferation function, and B lymphocytes may be more sensitive to PFOS than T lymphocytes.