用分光光度计预测小球藻藻液的浓度

Different concentrations of Chlorella vulgaris Beij were tested by 721 Visible Spectrophotometer under 41 groups of wave bands to get the light absorbance of algae. So the optimal wave band used to produce Chlorella could be checked out. The results showed that the optimal wave band is 670nm. This was due to the transmitted light intensity of algae got to the peak under 670 nm of incident light. The relationship between the light absorbance and the concentration of algae is, y=-1.4797+0.987x. The relativity between them equals 0.991. It shows they have good relativity. The formulate of the algae concentration (C) and the transmitted light intensity (I) is, Ｃ= -㏑（I/I0）/(LK). Where L is the width of the vessel and K is the section area of an algae cell.