针刺对2型糖尿病大鼠骨胳肌GLUT4mRNA的影响1

Objective: To research the effects of acupuncture on the expression of skeleton muscle GLUT4mRNA in type 2 diabetes mellitus(T2DM) model rats and the comparison of regulating action on expression of skeleton muscle GLUT4mRNA in T2DM rats between acupuncture and Glyburide. Method: 50 of 100 SD male rats fed with high fat feedstuff became fat rats, which were injected with Streptozotocin(25mg/kg) to make T2DM model. Then T2DM rats(18) were Stochastically devided into Glyburide group(6), acupuncture group(6) and model group(6). After treatment for 4 weeks, fast blood sugar(FBS) was examined by active blood sugar meter and fast insulin(FINS) was examined by radioimmunoassav(RIA) and expression of skeleton muscle GLUT4mRNA was examined by In Situ Hybridization(ISH), the results were compared with normal rats(6). Result: After treatment, the level of FBS in acupuncture and Glyburide group are obviously lower than model group(P<0.05); the level of FINS in acupuncture is markedly lower than model group(P<0.05), the level of FINS in Glyburide group is also lower than model group but there has no statistical meaning between them(P>0.05); expression of GLUT4mRNA in acupuncture and Glyburide group are obviously higher than model group(P<0.01); expression of GLUT4mRNA in Glyburide group is lower than acupuncture group(P<0.01). Conclusion: Acupuncture and Glyburide also can improve the expression of skeleton muscle GLUT4mRNA in T2DM rats, but acupuncture’s effect is better than Glyburide.