自体移植静脉中sonic hedgehog表达的实验研究
he sonic hedgehog is upregulated in autogenous vein grafts and correlated with the proliferation of vascular smooth muscle cells
目的 研究细胞周期相关因子sonic hedgehog(shh)在大鼠自体移植静脉中的表达变化.方法 wistar大鼠30只，建立颈静脉-腹主动脉移植模型,分为对照组、术后14天组及28天组。应用Real-time RT-PCR定量检测shh mRNA在移植血管中的表达变化。结果 Real-time PCR结果显示血管移植术后2、4周，shh mRNA表达显著增加。结论 自体静脉移植术后，shh在新生内膜中高表达并与VSMC增殖相关。
objective To study the expression of cell cycle related factor shh in autogenous vein graft. Method Autogenous vein graft model were established in 30 wistar rats by transplanting the left jugular vein to intra renal abdominal aorta with microsurgical technique.Graft veins were harvest at 14、28 days after transplantation. shh mRNA was measured by quatitative Real-time-RT-PCR. Results The mRNA of shh was upregulated in the graft vein. Conclusion sonic hedgehog is upregulated in autogenous vein grafts.
段志泉、张健、惠林萍、崔泽实、赵蕾、杨昱、李凤贺、辛世杰
基础医学生物科学研究方法、生物科学研究技术
移植静脉shh血管平滑肌细胞再狭窄
graft veinsonic hedgehogVSMCrestenosis
段志泉,张健,惠林萍,崔泽实,赵蕾,杨昱,李凤贺,辛世杰.自体移植静脉中sonic hedgehog表达的实验研究[EB/OL].(2010-01-19)[2025-07-16].http://www.paper.edu.cn/releasepaper/content/201001-774.点此复制
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