The ethanol tolerance in Saccharomyces cerevisiae under a phenomics perspective
The ethanol tolerance in Saccharomyces cerevisiae under a phenomics perspective
Abstract Ethanol (EtOH) is a substantial stressor for Saccharomyces cerevisiae. Data integration from strains with different phenotypes, including EtOH stress-responsive lncRNAs, are still not available. We covered these issues seeking systems modifications that drive the divergences between higher (HT) and lower (LT) EtOH tolerant strains under their highest stress conditions. We showed that these phenotypes are neither related to high viability nor faster population rebound after stress relief. LncRNAs work on many stress-responsive systems in a strain-specific manner promoting the EtOH tolerance. Cells use membraneless RNA/protein storage and degradation systems to endure the stress harming, and lncRNAs jointly promote EtOH tolerance. CTA1 and longevity are primer systems promoting phenotype-specific gene expression. The lower cell viability and growth under stress is a byproduct of sphingolipids and inositol phosphorylceramide dampening, acerbated in HTs by sphinganine, ERG9, and squalene overloads; LTs diminish this harm by accumulating inositol 1-phosphate. The diauxic shift drives an EtOH buffering by promoting an energy burst under stress, mainly in HTs. Analysis of mutants showed genes and lncRNAs in three strains critical for their EtOH tolerance. Finally, longevity, peroxisome, energy and lipid metabolisms, RNA/protein degradation and storage systems are the main pathways driving the EtOH tolerance phenotypes.
Wolf Ivan Rodrigo、Labate Carlos Alberto、Junior Ary Fernandes、Ramos ¨|rica、Martins Cesar、Valente Guilherme Targino、Marques Lucas Farinazzo、de Almeida Lauana Foga?a、de Moraes Leonardo Naz¨￠rio、Cardoso Luiz Henrique、Cataldi Thais Regiani、Fioretto Matheus Naia、Sim¨-es Rafael Plana、Grotto Rejane Maria Tommasini、Furtado Fabiana Barcelos、Schnepper Amanda Piveta、Nakajima Rafael Takahiro、Almeida Rodrigo Oliveira、Justulin Lu¨as Antonio、de Assis Golim Marjorie、L¨￠zari Lucas Cardoso、de Oliveira Alves Camila Cristina、Labate M?nica Veneziano、Nijland Jeroen G.、Driessen Arnold J. M.、Coan Rafael Luiz Buogo、Pinto Camila Moreira
Department of Bioprocess and Biotechnology. School of Agriculture. S?o Paulo State University (UNESP)||Department of Structural and Functional Biology, Institute of Biosciences. S?o Paulo State University (UNESP)Laborat¨?rio Max Feffer de Gen¨|tica de Plantas, Escola Superior de Agricultura Luiz de Queiroz, Universidade de S?o Paulo (USP)Laboratory of Bacteriology, Department of Chemical and Biological Sciences, S?o Paulo State University (Unesp), Institute of BiosciencesDepartment of Structural and Functional Biology, Institute of Biosciences. S?o Paulo State University (UNESP)Department of Structural and Functional Biology, Institute of Biosciences. S?o Paulo State University (UNESP)Department of Bioprocess and Biotechnology. School of Agriculture. S?o Paulo State University (UNESP)||Max Planck Institute for Heart and Lung ResearchDepartment of Bioprocess and Biotechnology. School of Agriculture. S?o Paulo State University (UNESP)Department of Bioprocess and Biotechnology. School of Agriculture. S?o Paulo State University (UNESP)||Laboratory of Applied Biotechnology, S?o Paulo State University (UNESP), Clinical Hospital of the Medical SchoolDepartment of Bioprocess and Biotechnology. School of Agriculture. S?o Paulo State University (UNESP)Department of Bioprocess and Biotechnology. School of Agriculture. S?o Paulo State University (UNESP)Laborat¨?rio Max Feffer de Gen¨|tica de Plantas, Escola Superior de Agricultura Luiz de Queiroz, Universidade de S?o Paulo (USP)Department of Structural and Functional Biology, Institute of Biosciences. S?o Paulo State University (UNESP)Department of Bioprocess and Biotechnology. School of Agriculture. S?o Paulo State University (UNESP)Department of Bioprocess and Biotechnology. School of Agriculture. S?o Paulo State University (UNESP)||Laboratory of Applied Biotechnology, S?o Paulo State University (UNESP), Clinical Hospital of the Medical SchoolLaboratory of Applied Biotechnology, S?o Paulo State University (UNESP), Clinical Hospital of the Medical SchoolDepartment of Bioprocess and Biotechnology. School of Agriculture. S?o Paulo State University (UNESP)Department of Structural and Functional Biology, Institute of Biosciences. S?o Paulo State University (UNESP)Instituto Federal de Educa??o, Ci¨oncia e Tecnologia do Sudeste de Minas Gerais - Campus Muria¨|Department of Structural and Functional Biology, Institute of Biosciences. S?o Paulo State University (UNESP)Laboratory of Applied Biotechnology, S?o Paulo State University (UNESP), Clinical Hospital of the Medical SchoolDepartment of Bioprocess and Biotechnology. School of Agriculture. S?o Paulo State University (UNESP)||Biomedical Sciences Institute, Department of Parasitology. University of S?o Paulo (USP)Department of Bioprocess and Biotechnology. School of Agriculture. S?o Paulo State University (UNESP)Laborat¨?rio Max Feffer de Gen¨|tica de Plantas, Escola Superior de Agricultura Luiz de Queiroz, Universidade de S?o Paulo (USP)Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute and Zernike Institute for Advanced Materials, University of GroningenMolecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute and Zernike Institute for Advanced Materials, University of GroningenDepartment of Biophysics and Pharmacology. Institute of Biosciences. S?o Paulo State University (UNESP)Department of Bioprocess and Biotechnology. School of Agriculture. S?o Paulo State University (UNESP)
分子生物学遗传学生物化学
OMICsdata integrationsystems biologylncRNAslncRNA-protein interactionsmembranelessCRISPR-Cas9
Wolf Ivan Rodrigo,Labate Carlos Alberto,Junior Ary Fernandes,Ramos ¨|rica,Martins Cesar,Valente Guilherme Targino,Marques Lucas Farinazzo,de Almeida Lauana Foga?a,de Moraes Leonardo Naz¨￠rio,Cardoso Luiz Henrique,Cataldi Thais Regiani,Fioretto Matheus Naia,Sim¨-es Rafael Plana,Grotto Rejane Maria Tommasini,Furtado Fabiana Barcelos,Schnepper Amanda Piveta,Nakajima Rafael Takahiro,Almeida Rodrigo Oliveira,Justulin Lu¨as Antonio,de Assis Golim Marjorie,L¨￠zari Lucas Cardoso,de Oliveira Alves Camila Cristina,Labate M?nica Veneziano,Nijland Jeroen G.,Driessen Arnold J. M.,Coan Rafael Luiz Buogo,Pinto Camila Moreira.The ethanol tolerance in Saccharomyces cerevisiae under a phenomics perspective[EB/OL].(2025-03-28)[2025-08-02].https://www.biorxiv.org/content/10.1101/2021.08.04.455136.点此复制
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