耐甲氧西林金黄色葡萄球菌的交叉引物扩增方法（CPA）检测

Objective: In this study, to detect methicillin resistant Staphylococcus aureus, through the comparison with traditional wafer inhibition ring test method, an efficient cross-priming amplification molecular identification method is established targeting on the mecA gene. Methods: 315 cases of Staphylococcus aureus from different clinical sources were used for the determination of the traditional paper and the identification of the new CPA method. The accuracy and sensitivity of the new method were determined. Results: The study showed that in all 315 cases of clinical Staphylococcus aureus, traditional methods of detecting results for 148 strains (46.98%) Staphylococcus aureus with methicillin resistance, however, CPA molecular method identification of 143 strains (45.40%). CPA method could complete the identification of bacterial resistance in only 1 hour, which greatly reduced the detection time of traditional methods, and provided a solid foundation for shortening the time of patient testing and improving the efficiency of medical treatment.