白菜WRKY转录因子cDNA片段的克隆及分析
loning of partial sequence of WRKY transcription factor gene in Brassica campestris ssp.chinensis.
根据GenBank中登录的拟南芥WRKY转录因子的保守序列设计引物，采用RT-PCR和3’-RACE方法，从SA处理的不结球白菜抗病自交系苏州青中，获得了473bp的 cDNA片断。序列分析表明，该序列含有WRKY转录因子共有的WRKYGQK保守结构域，与拟南芥AtWRKY18 氨基酸序列的同源性为83%，与拟南芥AtWRKY60同源性为72%，表明已经成功克隆了不结球白菜WRKY1基因3’末端cDNA序列，在GenBank中登录号为AF518555。
PCR primers were designed based on the conserved domain of Arabidopsis WRKY transcription factors. Using RT-PCR and 3’-RACE technology, the 473bp fragment of WRKY1 gene was obtained from 4h after SA treatment of total RNA isolated from Brassica campestris ssp.chinensis. The deduced amino acid sequence of this fragment contain the conserved WRKYGQK sequences, and further comparison showed that identity was 83% and 72% to AtWRKY18 gene and AtWRKY60 gene of Arabidopsis, respectively. As a result, the sequence was accepted and released by GenBank (accession number AY518555).
史公军、王彦华、侯喜林
分子生物学植物学遗传学
不结球白菜水杨酸WRKY转录因子RT-PCRRACE
Brassica campestris ssp.chinensissalicylic acidWRKY transcription factorRT-PCRRACE
史公军,王彦华,侯喜林.白菜WRKY转录因子cDNA片段的克隆及分析[EB/OL].(2004-06-03)[2025-08-02].http://www.paper.edu.cn/releasepaper/content/200406-13.点此复制
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