Colonization of intervertebral discs by Cutibacterium acnes in patients with low back pain: protocol for an analytical study with microbiological, phenotypic, genotypic, and multiomic techniques
Colonization of intervertebral discs by Cutibacterium acnes in patients with low back pain: protocol for an analytical study with microbiological, phenotypic, genotypic, and multiomic techniques
Abstract Lumbar disc degeneration (LDD) and low back pain (LBP) are two conditions that are closely related. Several studies have shown Cutibacterium acnes colonization of degenerated discs, but whether and how these finding correlates with LBP is unknown. A prospective study was planned to identify molecules present in lumbar intervertebral discs (LLIVD) colonized by C. acnes in patients with LDD and LBP and correlate them with their clinical, radiological, and demographic profiles. The clinical manifestations, risk factors, and demographic characteristics of participants undergoing surgical microdiscectomy will be tracked. Samples will be isolated and pathogens found in LLIVD will be characterized phenotypically and genotypically. Whole genome sequencing (WGS) of isolated species will be used to phylotype and detect genes associated with virulence, resistance, and oxidative stress. Multiomic analyses of LLIVD colonized and non-colonized will be carried out to explain not only the pathogen’s role in LDD, but also its involvement in the pathophysiology of LBP. This study was approved by the Institutional Review Board (CAAE 50077521.0.0000.5258). All patients who agree to participate in the study will sign an informed consent form. Regardless of the study’s findings, the results will be published in a peer-reviewed medical journal. Trials registration number NCT05090553; pre-results.
de Oliveira Ferreira Eliane、de Farias Gabriel Corr¨oa、Martha Antunes Luis Caetano、Cassiano Keila Mara、Fiorelli Rossano Kepler Alvim、Nogueira F¨￠bio C¨|sar Sousa、Magno da Rocha Vin¨acius、Ormundo Gon?alves Ximenes Lima Carla
Departamento de Microbiologia M¨|dica, Institito de Microbiologia Paulo de G¨?es, Universidade Federal do Rio de Janeiro (UFRJ)Departamento de Biologia Celular e Molecular, Funda??o Oswaldo CruzCentro de Desenvolvimento Tecnol¨?gico em Sa¨2de, Funda??o Oswaldo CruzDepartamento de Estat¨astica, Instituto de Matem¨￠tica, Universidade Federal Fluminense (UFF)Departamento de Cirurgia geral e especializada, Escola de Medicina, Universidade Federal do Estado do Rio de Janeiro (UniRio)Instituto de Qu¨amica, Universidade Federal do Rio de Janeiro (UFRJ)Departamento de Cirurgia geral e especializada, Escola de Medicina, Universidade Federal do Estado do Rio de Janeiro (UniRio)Departamento de Microbiologia M¨|dica, Institito de Microbiologia Paulo de G¨?es, Universidade Federal do Rio de Janeiro (UFRJ)
医学研究方法基础医学微生物学
Intervertebral discsCutibacterium acneslow back painanalytical studymultiomic techniques
de Oliveira Ferreira Eliane,de Farias Gabriel Corr¨oa,Martha Antunes Luis Caetano,Cassiano Keila Mara,Fiorelli Rossano Kepler Alvim,Nogueira F¨￠bio C¨|sar Sousa,Magno da Rocha Vin¨acius,Ormundo Gon?alves Ximenes Lima Carla.Colonization of intervertebral discs by Cutibacterium acnes in patients with low back pain: protocol for an analytical study with microbiological, phenotypic, genotypic, and multiomic techniques[EB/OL].(2025-03-28)[2025-05-18].https://www.medrxiv.org/content/10.1101/2022.07.08.22277406.点此复制
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