大黄素对3T3-L1前脂肪细胞增殖分化及相关基因表达的影响

im To explore the effect and mechanism of emodin (Em) on proliferation and differentiation of murine 3T3-L1 preadipocytes, and investigate its possible mechanism. Methods 3T3-L1 preadipocytes were cultured and treated with Em different dosages. Cell proliferation was analyzed by MTT method. Oil red O staining method and spectrophotography were applied to analyze the degree of differentiation. Real-time PCR was applied to detect the mRNA expression of peroxisome proliferator activated receptor γ(PPARγ) and CCTTA enhancer binding protein α(C/EBPα). Result Em could inhibit the proliferation of 3T3-L1 preadipocytes in different degrees. Em significantly inhibited the differentiation of 3T3-L1 preadipocytes and decreased the accumulation of lipid droplets in 20, 40 and 80 μM groups. The expression of PPARγ, C/EBPα, FAS and aP2 mRNA in adipocytes of 3T3-L1 cells were significantly decreased by Em 40 and 80μM compared with adipocytes. Conclusion Em could inhibit the differentiation of 3T3-L1 adipocytes and decrease the intracellular TG content. The mechanism might be related to down-regulating the expression of adipose differentiation-related genes PPARγ, C/EBPα, FAS and aP2 mRNA expression.