黄芪对低氧环境下SDF-1诱导hBMSCs迁移的干预作用的研究
he study on the intervention of Astragalus on the role of SDF-1 which induced hBMSCs to migrate to the lesion sites in hypoxic environment
目的]本研究模拟人体内低氧环境，研究黄芪和VEGF、SDF-1重要细胞因子对hBMSCs向VECs分化及迁移能力的影响。[方法]随机分为7组，将hBMSCs细胞悬液放入Transwell系统的上室，将含不同药物的细胞培养液加入到Transwell系统的下室中。将常氧三组Transwell系统置于普通CO2细胞培养箱（21%O2）中；低氧四组Transwell系统置于三气培养箱（5%O2）中培养。培养48h后，取上室，酒精棉球擦掉上室膜上未迁移的细胞，95％酒精固定l0min，苏木素染色5min，随机选择3个连续显微镜视野(×200)计数迁移到上室膜下的细胞，取平均数。[结果]（1）在常氧培养三组中，SDF+黄芪+常氧组与其他两组相比较，显著升高（p<0.01，p<0.05）。（2）在低氧培养四组中，SDF+黄芪+低氧组与其他三组相比较，显著升高（p<0.01）；与SDF+低氧组相比较，低氧对照组显著升高（p<0.01）；低氧对照组与黄芪+低氧组无统计学意义（p>0.05）。（3）与SDF+黄芪+常氧组相比较，SDF+黄芪+低氧组组间显著升高（p<0.05）；SDF+常氧组与SDF+低氧组、黄芪+常氧组与黄芪+低氧组组间无统计学意义（p>0.05）。[结论]在常氧和低氧环境中黄芪与SDF-1联合使用可促进BMSCs迁移：而在低氧环境中，单独应用黄芪没有明显的促进作用。
Objective To study the effect on the capabilities of migrating to the lesion sites and differentiating into vascular endothelial cells influenced by Astragalus，VEGF and SDF-1 by simulating hypoxic environment of human body. Methods HBMSCs were randomly divided into 7 groups.hBMSC cell suspensions were placed into the upper chamber of the Transwell system，cell culture medium with different drugs into the lower chamber.The three normxic groups were placed in ordinary cell culture incubator (21% O2) ；The four hypoxic groups in the special cell culture incubator (5% O2).After cultured for 48h，we removed the upper chamber of the Transwell system，wiped off cells on the membrane of the upper chamber that did not migrate，immersed the membrane with 95% alcohol in l0min，stained cells with hematoxylin in 5min，at last randomly selected three consecutive microscope visual field(×200) to count cells below the membrane，taking the average. Results (1)Among the three normoxic groups，the ability of migration of hBMSCs in SDF+Astragalus+ Normoxia group was significantly higher than that of others (p<0.01，p<0.05).(2)Among the four hypoxic groups，the ability of migration of hBMSCs in SDF+Astragalus+hypoxia group was significantly higher than that of others (p<0.01)；the ability of migration of hBMSCs in SDF+Hypoxia group was significantly higher than that in hypoxic control group (p<0.01)；the ability of migration of hBMSCs in hypoxia+Astragalus group was not significantly higher than that of Hypoxic control group(p> 0.05).(3)The ability of migration of hBMSCs in SDF+Astragalus +Hypoxia group was significantly higher than that in SDF+Astragalus+Normoxia group(p<0.05)；The ability of migration of hBMSCs in SDF +Normoxia group was not significantly higher than that in SDF+ Hypoxia group (p<0.05)；The ability of migration of hBMSCs in Astragalus+Normoxia group was not significantly higher than that in Astragalus +Hypoxia group (p<0.05)；Conclusions In normoxic and hypoxic environment ,Astragalus combined with SDF-1 can promote the migration of BMSCs.In the hypoxic environment，only Astragalus can not promote the migration of BMSCs.
沈凌、林冬阳、杨博华
中医学基础医学细胞生物学
骨髓间充质干细胞黄芪基质细胞衍生因子-1迁移血管内皮细胞生长因子血管内皮细胞
BMSCsAstragalusMigrationSDF-1VEGFVECs
沈凌,林冬阳,杨博华.黄芪对低氧环境下SDF-1诱导hBMSCs迁移的干预作用的研究[EB/OL].(2012-03-20)[2025-08-10].http://www.paper.edu.cn/releasepaper/content/201203-589.点此复制
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