同时提取当归中阿魏酸松柏酯和藁本内酯：双水相与三液相的比较

ngelica sinensis (Oliv.) Diels exhibits a variety of pharmacological activities, including anti-cancer, anti-oxidation, cardiovascular protection and others. Coniferyl ferulate (CF) and ligustilide (Lig) are the main bioactive components of Angelica sinensis. Therefore, efficient extraction and separation of CF and Lig is of great significance. Newly rising aqueous two-phase system (ATPS) and three-liquid-phase system (TLPS) have the advantages of high extraction yield and good selectivity. Both ATPS and TLPS could be used in extraction of multi-components, but choice of ATPS or TLPS is a question for researchers. In this study, CF and Lig were simultaneously extracted by ethanol-NaH2PO4-H2O ATPS and cyclohexane-ethanol-NaH2PO4-H2O TLPS, and the extraction effect were compared between ATPS and TLPS. The optimal extraction conditions of TLPS were as follows: NaH2PO4 solution (3.75mol/L, pH=5.00), ethanol and cyclohexane volume fractions were 34.0%, 38.0% and 28.0%, respectively, extraction temperature was 45.0℃, extraction time was 2.00 h. For the extraction at 40.0℃ for 1.00 h with optimal NaH2PO4 solution (3.33mol/L, pH=5.00) volume fraction 47.8%. Compared to those with TLPS, the CF yield with ATPS and Lig yield with ATPS increased by 28.9% and 11.4% respectively. The reasons for good yields of ATPS will be further investigated.