玛曲县境内纤维素酶野生菌株的筛选及发酵条件的优化
Screening of cellulase-producing strains from MaQu County of China and Optimizing Culture Conditions
利用西北地区独特的自然资源，经过选择培养基和刚果红染色法初筛分离到61株能降解纤维素的菌株，再经摇瓶复筛得到5株纤维素分解能力比较强的菌株，最后挑出一株酶活最高的霉菌G7，并初步分析了其最适产酶条件：CMCNa最适浓度:0.4%，(NH4)2SO4:0.15%，最适温度28℃，pH6.0，发酵时间:4天，其PFA和CMCase分别达到1.767U/mL和7.524U/mL。
We got sixty-one strains from the MaQu county of China which had special climate by screening with transparent zone method.Then through shaking culture method,we got five high cellulase-producing stains.Finally,We chose the highest cellulase-producing strain named G7. Preliminary analysis of submerged fermentation showed that the process required the optimal concentration of CMCNa was 0.4%, (NH4)2SO4 0.15%,pH 6.0, and a temperature range between 28°C and 30°C,cultured for 4 days.
李红玉、李素波、周剑平
微生物学生物工程学生物化学
纤维素酶筛选发酵
cellulasescreeningfermentation
李红玉,李素波,周剑平.玛曲县境内纤维素酶野生菌株的筛选及发酵条件的优化[EB/OL].(2008-02-10)[2025-07-23].http://www.paper.edu.cn/releasepaper/content/200802-78.点此复制
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