Synaptic vesicles dynamics in neocortical epilepsy
Synaptic vesicles dynamics in neocortical epilepsy
Abstract Neuronal hyperexcitability often results from an unbalance between excitatory and inhibitory neurotransmission, but the synaptic alterations leading to enhanced seizure propensity are only partly understood. Taking advantage of a mouse model of neocortical epilepsy, we used a combination of photoconversion and electron microscopy to assess changes in synaptic vesicles pools in vivo. Our analyses reveal that epileptic networks show an early onset lengthening of active zones at inhibitory synapses, together with a delayed spatial reorganization of recycled vesicles at excitatory synapses. Proteomics of synaptic content indicate that specific proteins were increased in epileptic mice. Altogether, our data reveal a complex landscape of nanoscale changes affecting the epileptic synaptic release machinery. In particular, our findings show that an altered positioning of release-competent vesicles represent a novel signature of epileptic networks.
Vannini Eleonora、Restani Laura、Marra Vincenzo、Dilillo Marialaura、McDonnell Liam、Caleo Matteo
Neuroscience Institute, National Research Council (CNR)||Department of Neuroscience, Psychology and Behaviour, University of Leicester||Fondazione Umberto VeronesiNeuroscience Institute, National Research Council (CNR)Department of Neuroscience, Psychology and Behaviour, University of LeicesterFondazione Pisana per la Scienza Onlus (FPS), via Ferruccio Giovannini 13, San Giuliano TermeFondazione Pisana per la Scienza Onlus (FPS), via Ferruccio Giovannini 13, San Giuliano TermeNeuroscience Institute, National Research Council (CNR)||Department of Biomedical Sciences, University of Padua
神经病学、精神病学基础医学细胞生物学分子生物学
epilepsysynaptic vesicleshyperexcitabilityvisual cortexvisual processingtetanus neurotoxin
Vannini Eleonora,Restani Laura,Marra Vincenzo,Dilillo Marialaura,McDonnell Liam,Caleo Matteo.Synaptic vesicles dynamics in neocortical epilepsy[EB/OL].(2025-03-28)[2025-06-04].https://www.biorxiv.org/content/10.1101/2020.01.05.895029.点此复制
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