可溶性的meClpsl蛋白的原核表达和辅脂酶功能检测

Objective: To express meClpsl with prokaryotic system, verify whether it has colipase activity or not. Methods: The meClpsl gene was inserted into the prokaryotic expression vector pET32b, after induced by IPTG，the recomibinant meClps protein was expressed induced by IPTG and purified with affinity chromatography. The colipase kit was used to detect whether the meClps has the colipase activity. Result: The fusion meClpsl protein was soluble, but could not promote the hydrolysis of lipase to its substrate glycerine trioleate, similar as the negative control protein. Conclusion: It revealed that meClpsl protein had no effect on pancreatic lipase, and may not possess the colipase function.