陆地棉NAC家族全基因组鉴定及表达分析

Objective] Upland cotton is an important fiber and oil crop. Transcription factors of NAC(NAM, ATAF, CUC) gene family play an important role in plant growth, development and environmental adaptation, but the identification of NAC family members in upland cotton and the study of expression characteristics under biotic and abiotic stresses are not complete and sufficient. In recent years, with the development of genome sequencing technology, the number and expression characteristics of NAC family members need to be further clarified under the background of higher quality genome information and various transcriptome data. [Method] Based on the genome data of modern upland cotton variety Nongdamian No.8 and the transcriptome data in cotton public database, all members of NAC gene family in upland cotton were identified by bioinformatics method, and the physical and chemical properties, gene structure, evolution relationship of members, tissue expression specificity and expression law under stress were analyzed. [Result] The NAC gene family of upland cotton contains 306 members distributed on 26 chromosomes, which can be divided into 14 subgroups according to amino acid sequence homology. Most NAC family members have thermal stability and stable structure, and most of them are located in the nucleus. There are mainly light stress response and hormone-related cis-acting elements such as auxin and gibberellin in the NAC family gene promoter (upstream 2 kb) sequence. The analysis of tissue expression characteristics showed that the expression of most NAC genes in stamens or pistils was higher than other tissues. The expression analysis under adversity stress showed that 53 genes responded to cold, heat, salt or drought stress in cotton. Analysis of expression patterns under verticillium wilt stress showed that 44 genes responded to verticillium wilt stress in cotton. [Conclusion] The chromosome distribution, structural characteristics and phylogenetic relationship of NAC family in upland cotton were clarified, and the response characteristics of NAC family genes in cotton growth and development, biological stress and abiotic stress were revealed through transcriptome data.