尾加压素Ⅱ在人类肺纤维化中的表达及意义
Expression and Role of UⅡ in Pulmonary Fibrosis
目的 通过检测尾加压素Ⅱ（urotensin Ⅱ，UⅡ）在纤维化肺组织中的表达，探讨其在肺纤维化发生发展中的意义。方法 用免疫组化法检测UⅡ、α- 平滑肌肌动蛋白（α- smooth muscle actin , α-SMA）、纤维连接蛋白（fibronectin，FN）、Ⅲ型胶原在纤维化肺组织中的表达，用原位杂交法检测UⅡmRNA在纤维化肺组织中的分布。结果 与正常组织中UⅡ极少量分布在成纤维细胞相比，纤维化肺组织中主要分布于成纤维细胞和平滑肌细胞。与正常对照组（stage 0，S0）相比，轻度(stage 1，S1)、中度(stage 2，S2)和重度(stage 3，S3)纤维化组的UⅡ蛋白表达阳性率差异均有显著性（P=0.005、P=0.007、P=0.005）。肺纤维化程度与UⅡmRNA、蛋白质的表达强度均呈正相关(P=0.000、P=0.000)；UⅡ蛋白表达强度与α-SMA、FN的表达强度呈正相关(P=0.000、P=0.000)。结论 UⅡ参与了肺纤维化的发生发展。
Objective To study the expression of UⅡ in pulmonary fibrosis for disclosing the role of UⅡ in development of pulmonary fibrosis. Methods The expressions of UⅡ, α-SMA, FN, and collgen type Ⅲ in tissues of pulmonary fibrosis were observed by immunohistochemical technique. Distribution of UⅡmRNA in tissues of pulmonary fibrosis was localized by in situ hybridization. Results UⅡwas mainly expressed in fibroblasts and leiomyocytes of fibrotic lung tissue, while weakly expressed in fibroblasts of normal lung tissue. In contrast to control, there were significant differences of positive rates in pulmonary fibrosis stage1, 2 and 3 (P=0.002, P=0.007,P=0.002).The intensitivities of UⅡmRNA and protein pulmonary fibrosis (P=0.000, P=0.000). The intensitivities of α-SMA and FN were showed to be positively correlated to that of UⅡ protein(P=0.000,P=0.000). Conclusion It is suggested that UⅡ be implicated in the development of pulmonary fibrosis.
薛永杰、王平凡、贺雪娇、杨国嵘、郁荣、朱任之
基础医学
肺纤维化UⅡ免疫组化原位杂交
Pulmonary FibrosisUⅡImmunohistochemistryIn situ hybridization.
薛永杰,王平凡,贺雪娇,杨国嵘,郁荣,朱任之.尾加压素Ⅱ在人类肺纤维化中的表达及意义[EB/OL].(2008-03-21)[2025-08-10].http://www.paper.edu.cn/releasepaper/content/200803-634.点此复制
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