人多能干细胞体外向间充质样细胞分化的研究进展

Mesenchymal stem cells, as one of the seed cells for tissue engineering, have been widely used in various aspects like autoimmune, tumor treatment, injuries and so on. However, a number of factors such as inadequate numbers of cells obtained from tissue biopsies, limited extension times and impaired growth and function. Additionally, the differentiation ability of MSCs are impaired by aging and aging-related diseases. Because of the unlimited self-renewal and multi-differentiation potential in vitro, human pluripotent stem cells (hPSCs) are becoming the most promising source of seed cells for tissue engineering and cell therapy. Recently, many researchers have found that human pluripotent stem cells derived mesenchymal stem cells (hPSCs-MSCs) have similar properties to adult mesenchymal stem cells. Compared with adult mesenchymal stem cells, hPSCs-MSCs also have the advantages of stronger proliferation ability, unlimited cell number, and lower immunogenic risk. But at the same time, it also has problems such as unclear components, low induction efficiency, and unclear identification standards, which need to be explored and solved by researchers. The differentiation, identification and purification methods of hPSCs-MSCs was reviewed, and we also point out the problems and deficiencies in current applications, and prospect their application, in order to promote the clinical application of hPSCs-MSCs.