水稻OsTPR1蛋白与乙烯受体蛋白OsERS1相互作用的酵母双杂交技术检测
etermination of interaction between OsTPR1 and OsERS1 by yeast two-hybrid system
OsTPR1是水稻TPR蛋白家族的重要成员之一，可能参与了乙烯信号转导。本文构建了酵母共表达载体pGBKT7-OsTPR1和pGADT7-OsERS1、pGADT7-OsERS1(952-1359)、pGADT7-OsERS1(1360-1908)载体，共转酵母AH109。检测结果表明，OsTPR1与OsERS1具有相互作用，且关键域为OsERS1(952-1359)和OsERS1(1360-1908)，这为深入探讨OsTPR1蛋白在水稻乙烯应答反应中的作用及分子基础提供了依据。
OsTPR1 was a important member of TPR proteins in rice, may participate in the ethylene signal transduction. Theyeast expression vector pGBKT7-OsTPR1 and pGADT7-OsERS1, pGADT7-OsERS1(952-1359), pGADT7-OsERS1(1360-1908) were constructed successfully, and then co-transformed into AH109 yeast cells. The yeast two-hybird system detected the interaction between OsTPR1 and OsERS1. The OsERS1 domain OsERS1(952-1359) and OsERS1(1360-1908) were identified to play key role in the intereaction between OsTPR1 and OsERS1. OsTPR1 participated in ethylene signaling transduction pathway associated with OsERS1.
王佳、田云、谌惠邦、卢向阳、易文凯、杨辉
分子生物学细胞生物学植物学
分子生物学水稻PR蛋白蛋白相互作用酵母双杂交技术
Molecular biologyRicePR proteinProtein interactionYeast two-hybird system
王佳,田云,谌惠邦,卢向阳,易文凯,杨辉.水稻OsTPR1蛋白与乙烯受体蛋白OsERS1相互作用的酵母双杂交技术检测[EB/OL].(2017-04-18)[2025-08-03].http://www.paper.edu.cn/releasepaper/content/201704-178.点此复制
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