玉米AFB2基因转化番茄的功能研究

A homologue of AFB2 gene, ZmAFB2 (GenBank: NM_001143136.1), has been cloned using the gene special primer from maize (Zea mays L.). It contains a 1,722 bp open reading frame (ORF) encoding 574 amino acid residues. Quantitative PCR analysis showed that ZmAFB2 expressed in all tissue in maize, including root, stem and leaf, but mainly in stem. To further investigate the function of ZmAFB2, we constructed ZmAFB2 over-expression vector pCAMBIA-35s-AFB2, and transformed into tomato (Solanum lycopersicum) by Agrobacterium-mediated transformation and through the identification of GUS stain and quantity PCR, we got three transgenic tomato lines(line2,line3 and line4). While line2 and line4 were no seeds, quantitative PCR showed IAA9 and ARF8 expression levels were declined, whereas ARF2 expression up-regulated. Our result indicated that ZmAFB2 may take part in another pathway in auxin regulation different from TIR1, and have an important role in fruit development by activating the auxin response factor 2 (ARF2).