小鼠1号染色体Zdbf2-Gpr1印记区1个EST的印记鉴定及表达分析

In mammals in particular, imprinted genes play important roles in fetal growth, development and maternal behavior. Abnormal expression of imprinted genes is implicated in many diseases including cancer. Therefore, a study of genomic imprinting is interesting for not only development of the theory of developmental biology and epigenetics, also treatment and diagnosis of related diseases. The primary task of the study of genomic imprinting is the identification of new imprinted genes and analysis of their expression pattern. Therefore, the study analysed imprinting status of an EST CJ051953 in Zdbf2-Gpr1 imprinting domain on chromosome 1 as candidate imprinted genes based on clusters features of imprinted genes by direct sequencing of SNPs approach. Fothermore, the study analysed expression patterns of the candidate imprinted genes by in situ hybridization (ISH). Imprinting analysis showed preferential paternally expressed of CJ051953 in the mouse. Expression analysis showed that CJ051953 was highly and continuously expressed in nervous system, which suggested that the EST may be involved in the mouse embryonic nervous system development.