苦荞 FtF5H 基因克隆及表达分析

he Convolvulaceae is a pantropical family with high morphological diversity and economic values. To date, however, the phylogenetic relationships among the major lineages or tribes in the family have not been fully resolved. In order to resolve these relationships, we sampled the complete plastome sequences from 40 species, representing eight recognized tribes of Convolvulaceae, and reconstructed phylogenetic trees by using both Maximum Likelihood and Bayesian Inference approaches. As the main results, we found that: (1) Convolvulaceae plastomes have the typical quadripartite structure, with the genome size ranging from 113 273 bp to 164 112 bp and including 66-79 protein-coding genes. (2) Phylogenomic analyses using five datasets (i.e., WCG, CDS, LSC, IR, and SSC matrixes) show that the topologies of the WCG and CDS trees are identical, with differences in support values for some nodes. Topological differences were found between LSC and WCG topologies, especially for the positions of Cuscuteae, Dichondreae, and Cresseae. The AU and SH tests show that topological conflicts are significant between the WCG tree and the SSC and IR trees. (3) All phylogenetic analyses confirm that Cuscuta and Dichondreae are nested in Convolvuloideae and should be treated as tribes. (4) Phylogenetic relationships among the eight tribes are well resolved using the WCG and CDS datasets: Cardiochlamyeae and Erycibeae form a clade as the first divergent group of Convolvuloideae, followed by Cuscuteae, with the remaining five tribes forming two major clades. (6) The phylogenomic analyses confirm that Merremieae, and in particular the genus Merremia, are polyphyletic, and that the circumscription and taxonomy of both Merremieae and Merremia need revision.