miR-680可促进Runx2诱导C2C12细胞进行成骨细胞早期分化

To investigate the role of miR-680 during Runx2-induced osteoblast differentiation in C2C12 cells. Methods:We used Tet-on inducible gene expression system and established C2C12/Runx2Dox sub-line with conditional Runx2 expression induced by inducer Doxycyclin(Dox) in mouse myoblast C2C12.Firstly,we studied the miR-680 expression induced by Runx2 at different time points by Real-time PCR. Dox induced C2C12/Runx2Dox to the process of osteogenic differentiation in vitro transfection miR-680.Detected the activity of ALP in osteoblast by alkaline phosphatase (ALP) staining and Real-time PCR.We prediction miRNA-680 target gene by online database miRanda, miRwalk, Targetscan and function classification.Results and conclusion:The expression of miR-680 was significantaly highly in osteoblast differentiation.Transfection miR-680 mimics into cells enhanced the activity of alkaline phosphatase and Real-time PCR results showed that the expression of Alp was significantly increased. Results suggested that miR-680 was highly expressed in C2C12/Runx2Dox to the osteogenic differentiation process and can promote the early differentiation into bone.