重金属胁迫对水稻粳籼亚种特定序列DNA甲基化的影响

Under heavy metal stress, plants would regulate their corresponding physiological progresses such as photosynthesis, respiration, antioxidant system and hormone metabolism, etc. Recent years a growing numbers of research suggests that epigenetic regulations involved in the plant coping with different kinds of stress response, however, the detain mechanisms involved in the stress response are still not clear, some further studies show that epigenetic mechanism involves in the plants coping with stress and which is necessary for the future application in plant breeding and conservation. In this study, the japonica rice "Nipponbare" and indica rice "9311" were used as materials, then treated them with heavy metals, using Southern blot method, we detected the DNA methylation changes of special sequences including transposons and low copy cellular genes in treated rice plants compared with the Mock plants, the results showed that japonica rice "Nipponbare" occurred specific hypomethylation and predominately in CHG sites in most of the investigated sequences, while indica rice "9311" kept stable in all investigated sequences, suggesting that epigenetic mechanism involved in rice subspecies coping with heavy metal stress may be different.