halidomide通过保护软骨下骨延缓小鼠DMM骨关节炎

im: To investigate the effects of thalidomide on cartilage and subchondral bone in DMM model of osteoarthritis in mice, and to provide evidence for its role in the development of osteoarthritis. Methods: The C57/BL6 mice were randomly divided into experimental group, control group and sham operation group, DMM method was used on the mice of experimental group and the control group to establish the model of knee osteoarthritis, the sham operation group merely cut the skin and joint capsule in the corresponding position of the right knee joint. The experimental group was injected intraperitoneally with thalidomide, and the control group and the sham operation group were injected with normal saline according to the same standard. MMP-13 immunofluorescence, Safranin O-Fast green staining, micro-CT and HE staining were used to detect the changes in cartilage and subchondral bone. Results: At 2 weeks, the changes in subchondral bone in experimental group and sham operation group significantly less than the control group. At 4 weeks, the subchondral bone and articular cartilage in the experimental group were significantly less than those in the control group. Conclusion: Thalidomide can reduce the pathological changes of subchondral bone in DMM osteoarthritis model, and may play an important role in delaying the development of osteoarthritis.