乌司他丁对大鼠肾脏机械性损伤后肾脏HSP70、IL-6表达的早期影响

IM&nbsp;&nbsp; To investigate the significance of ulinastatin’s effects on expression of IL-6 ,HSP70&nbsp; in&nbsp; kidney after mechanical injury. METHODS&nbsp;&nbsp; Sixity-six adult female Wistar rats were randomly divided into three groups: control(C, n=6), trauma(T, n=30), and injected ulinastatin after trauma(U, n=30). The model of trauma rats was made with free-fall bio-impactor striking the spinal column and ribs of rats. The expression of IL-6 and HSP70 in each group’s renal tissue&nbsp; were detected with tissue chip and immunohistochemical stain at 1，6, 12, 24 and 36 h after trauma. RESULTS&nbsp;&nbsp; The expression of IL-6 and HSP70 in renal tissue was low in control group. Compared with control, the expression of IL-6 was markedly increased at 1, 6, 12, 24 h hafter trauma (P<0.05),the expression of HSP70 in trauma group was significantly increased at 6 h after trauma(P<0.05).The expression of IL-6&nbsp; in&nbsp; group injected ulinastatin after trauma&nbsp; were more lower than trauma group, the expression of HSP70 at 1, 24, 36 h after trauma was higher than trauma group especially at 1 h(P<0.05). CONCLUSION&nbsp;&nbsp; Ulinastatin&nbsp; can reduce the risk of renal injury in early times after trauma especially at 1 hour. The protective effects of ulinastatin&nbsp; associated&nbsp; with the decrease of IL-6 expression, and up-regulation of&nbsp; HSP70 expression.