过氧化氢促进氯化钆诱导HepG2细胞凋亡

nticancer gadolinium compounds are of great interest in the studies of medical applications of lanthanides. Investigations on the mechanisms of anticancer actions of gadolinium compounds revealed that gadolinium decreased the levels of reactive oxygen species (ROS) in cancer cells, which is inconsistent with the current view of ROS in cell apoptosis. The present work investigated the roles of hydrogen peroxide on gadolinium induced apoptosis of HepG2 cancer cell line. The experimental results revealed that hydrogen peroxide promote gadolinium-induced HepG2 apoptosis. Analyses of apoptotic relevant proteins with Western Blot indicated that incubation of cells with Gd(III) ions and hydrogen peroxide results in significant cleavage PARP proteins but caused much weaker effects on MAPK or mitochondrial apoptotic signal transduction. Meanwhile, Gd(III) ions and hydrogen peroxide could cause fragmentations of calf thymus DNA. All these results suggested that instead of enhancing Gd-induced cellular signal, hydrogen peroxide promotes HepG2 apoptosis by increasing Gd-induced DNA damages. Further works into the mechanisms would be appropriate, nevertheless, this mechanism may provide new insight for design and discovery of anticaner lanthanide compounds.