紫杉醇抑制Hut78细胞生长及诱导凋亡的研究
Study on the effects of paclitaxel on the growth and apoptosis induction in Hut78 cell line
目的 研究紫杉醇对皮肤T细胞淋巴瘤（cutaneous T cell lymphoma, CTCL）细胞株Hut78的生长抑制及凋亡诱导作用，并探讨其诱导凋亡作用的机制。方法 紫杉醇不同浓度、不同作用时间分别处理Hut78细胞，应用端粒酶重复序列扩增及酶联免疫吸附方法（TRAP-PCR-ELISA）检测端粒酶活性的变化；采用形态学观察和流式细胞仪技术（FCM）检测其诱导Hut78细胞凋亡的情况；四甲基偶氮唑蓝法（MTT）测定Hut78细胞的生长抑制率。结果 紫杉醇明显下调端粒酶活性表达，对Hut78细胞具有生长抑制及诱导凋亡作用，且呈时间依赖和剂量依赖性。表现为G2/M期阻滞、出现典型的凋亡细胞特征，流式细胞仪检测，可见明显的凋亡峰。结论 紫杉醇能下调端粒酶的活性，诱导细胞凋亡是其发挥抗癌作用的机制之一。
Objective To observe the effects of apoptosis induction of paclitaxel on cutaneous T-cell lymphoma cell line Hut78 in vitro. Methods Hut78 was treated by paclitaxel with different concentration and acting time. Telomerase activity was determined by TRAP-PCR-ELISA. The inhibition of Hut78 cell growth was measured by MTT assay. The apoptosis of Hut78 was studied by flow cytometry. Results Paclitaxel could down-regulate telomerase activity, inhibit the growth of Hut78, and induce cell apoptosis in a dose- and time- dependent manner. S phase and G2/M phase DNA content increased and a typical subdiploid peak before G0/G1 phase was observed by flow cytometry. Conclusion Paclitaxel has obvious effects of growth-inhibiting on cutaneous T-cell lymphoma and can induce apoptosis of Hut78 cell.
孙建方、徐秀莲
肿瘤学基础医学药学
紫杉醇L细胞凋亡
PaclitaxelTelomeraseCutaneous T-cell LymphomaApoptosis
孙建方,徐秀莲.紫杉醇抑制Hut78细胞生长及诱导凋亡的研究[EB/OL].(2006-11-01)[2025-08-10].http://www.paper.edu.cn/releasepaper/content/200611-21.点此复制
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