硫氧还蛋白还原酶基因TRR1过表达对酿酒酵母抗氧化特性的影响

hioredoxin reductase 1 (Trr1p) is one of the most important parts in the antioxidative system of Saccharomyces cerevisiae. It plays a significant role in maintaining cells' redox state. In this study, according to the oxidative stress tolerance mechanism, thioredoxin reductase gene TRR1 was cloned onto vector pYES2 to generate recombinant plasmid pYES2-trr1, by which TRR1 gene was overexpressed in S. cerevisiae. The effects of TRR1 gene overexpression were investigated under oxidative stress induced by H2O2 or menadione. The results showed that TRR1 gene overexpression could apparently enhance antioxidative ability of S. cerevisiae.