同种异体移植炎症因子1（AIF-1）对婴幼儿血管瘤的作用及其机制的研究
he potential roles of allograft inflammatory factor-1 in the pathogenesis of hemangiomas: AIF-1 promotes the proliferation, migration and angiogenesis of human endothelial cells (HUV-EC-C) probably by up-regulation of bFGF
目的：本部分研究旨在通过探讨AIF-1对内皮细胞的作用，来探讨AIF-1对血管瘤发生发展中可能的作用存在机制；方法：构建AIF-1的表达质粒pcDNA3.1(-)-AIF-1转染正常血管內皮细胞系HUV-EC-Cs，建立AIF-1过表达的内皮细胞株；通过MTT、流式细胞周期实验检测内皮细胞的增殖，内皮细胞划痕及迁移实验检测内皮细胞的迁移能力，成管实验及鸡胚尿囊膜成血管实验内皮细胞的分化能力、半定量的RT-PCR、Real time PCR及ELISA实验方法检测血管内皮生长因子（vascular endothelial growth factor，VEGF）、碱性成纤维细胞生长因子（basic fibroblast growth factor，b-FGF）、金属蛋白酶组织抑制剂 1（tissue inhibitor of metalloproteinase 1，TIMP-1）、单核细胞趋化蛋白 1（monocyte chemoattractant protein-1，MCP-1）及粒细胞集落刺激因子（granulocyte colony-stimulating factor，G-CSF）的mRNA和蛋白水平；结果：正常内皮细胞不表达AIF-1，转染后AIF-1的表达显著增加；MTT及流式细胞周期实验显示内皮细胞表达AIF-1后增殖能力显著增强（p<0.05），停留于静止期（G0/G1）的细胞比例明显减少，而处于DNA合成期（S）及分裂期（G2/M）的细胞比例明显增加；细胞划痕及迁移实验同样证实AIF-1能促进内皮细胞迁移；同时，成管实验及鸡胚尿囊膜成血管实验显示AIF-1能促进血管生成；并且，AIF-1可以上调内皮细胞bFGF的表达，而对VEGF、TIMP-1、MCP-1及G-CSF的表达无影响；结论：AIF-1有可能是通过上调bFGF的表达来促进内皮细胞增殖、迁移及血管生成，从而最终影响血管瘤的发生发展。
Objective: To explore the impact of AIF-1 alterations on human endothelial cells (HUV-EC-C). Methods: Stable introduction of AIF-1 to human umbilical vein endothelial cell line (HUV-EC-C) in vitro was used to evaluate the role of AIF-1 by examining the change of cell proliferation, cell cycle, cell migration, angiogenesis and the expression level of proliferative and angiogenic related factors. Cell proliferation and cycle was measured by MTT assay and Flow Cytometry, Cell migration was assessed by the wound healing assay and the endothelial cell migration assay, and the ability of angiogenesis was investigated by the tuber formation assay and chicken chorioallantoic membrane assay. Furthermore, the expression levels of G-CSF, VEGF-a, bFGF MCP-1 and TIMP-1 was assessed by the semi-quantitative RT-PCR, Real time PCR and ELISA assay. Results: AIF-1 enhanced the proliferation,migration and angiogenesis of the endothelial cells and promoted G0/G1-to-S-phase transition, accompanied by up-regulation the expression of bFGF (P<0.05), while AIF-1 could not influence the expression of G-CSF, VEGF-a, MCP-1 and TIMP-1. Conclusions: Our studies demonstrated that AIF-1 could promote the proliferation, migration and angiogenesis of endothelial cells, probably through augmenting the expression of bFGF. The impact of AIF-1 on endothelial cells would stimulate angiogenesis, and consequently influence the progression of infantile hemangiomas.
赵怡芳、蔡育、贾俊
基础医学生物科学研究方法、生物科学研究技术肿瘤学
口腔颌面外科婴幼儿血管瘤同种异体移植炎症因子
oral and maxillofacial surgeryinfantile hemangiomasallograft inflammatory factor
赵怡芳,蔡育,贾俊.同种异体移植炎症因子1（AIF-1）对婴幼儿血管瘤的作用及其机制的研究[EB/OL].(2011-03-03)[2025-08-19].http://www.paper.edu.cn/releasepaper/content/201103-160.点此复制
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