Geobacter sulfurreducens微生物燃料电池产电过程的蛋白质组学分析

Microbial Fuel Cell is a new environment energy technology, while substance, energy and electron transfer processes in the process of electricity generation by microorganisms are not clear, limiting the MFC development. Previous studies found that Geobacter surferreducens can totally oxidize electron donor with electrode as the sole electron acceptor; and some proteins and genes related with electricity generation and electron transfer process via gene knockout and proteomic techniques. C-type cytochrome on the outermembrane may be the key substance during the process of transferring electrons to insoluble electron acceptors out of cells. However, researches on electricity generation process of MFC via proteomic strategies with anode microorganisms of G. sulfurreducens Fuel Cell as subject are rare. Differential proteins mainly participating substance and energy transportation, proton transfer and microorganism resilience in bacterial cells via proteomics methods were found. These proteins were in the bacterial outer membrane, periplasmic space, inner capsule and cytoplasm. Certain amount of potential sites of phosphorylation and glycosyl modification were found through postranslational modification forecast of differentially expressed proteins possibly containing various kinds of isomers. The figure of substance transport and electron transfer of G. sulfurreducensFC was drawn.)