Identification of Gli1 as a progenitor cell marker for meniscus development and injury repair
Identification of Gli1 as a progenitor cell marker for meniscus development and injury repair
Abstract Meniscal tears are associated with a high risk of osteoarthritis but currently have no disease-modifying therapies. Using Gli1-CreER tdTomato mice, we found that Gli1+ cells contribute to the development of meniscus horns from 2 weeks of age. In adult mice, Gli1+ cells resided at the superficial layer of meniscus and expressed known mesenchymal progenitor markers. In culture, meniscal Gli1+ cells possessed high progenitor activities under the control of Hh signal. Meniscus injury at the anterior horn induced a quick expansion of Gli1+ cells. Normally, the tissue healed slowly, leading to cartilage degeneration. Ablation of Gli1+ cells further hindered this repair process. Strikingly, intra-articular injection of Gli1+ meniscal cells or an Hh activator right after injury accelerated the bridging of the interrupted ends and attenuated signs of osteoarthritis. Taken together, our work identified a novel progenitor population in meniscus and proposes a new treatment for repairing injured meniscus and preventing osteoarthritis.
Liu X. Sherry、Zhang Yejia、Koyama Eiki、Long Fanxin、Qin Ling、Gui Tao、Zhong Leilei、Ahn Jaimo、Han Lin、Mauck Robert L、Zgonis Miltiadis、Sun Hao、Heo Su-Jin、Wei Yulong、Yu Wei、Yao Lutian
Department of Orthopaedic Surgery, Perelman School of Medicine, University of PennsylvaniaDepartment of Orthopaedic Surgery, Perelman School of Medicine, University of Pennsylvania||Translational Musculoskeletal Research Center, Corporal Michael J. Crescenz VA Medical Center||Department of Physical Medicine and Rehabilitation, Perelman School of Medicine, University of PennsylvaniaTranslational Research Program in Pediatric Orthopaedics, The Children?ˉs Hospital of PhiladelphiaTranslational Research Program in Pediatric Orthopaedics, The Children?ˉs Hospital of PhiladelphiaDepartment of Orthopaedic Surgery, Perelman School of Medicine, University of PennsylvaniaDepartment of Orthopaedic Surgery, Perelman School of Medicine, University of Pennsylvania||Department of Bone and Joint Surgery, Institute of Orthopedic Diseases, The First Affiliated Hospital, Jinan UniversityDepartment of Orthopaedic Surgery, Perelman School of Medicine, University of PennsylvaniaDepartment of Orthopaedic Surgery, Perelman School of Medicine, University of PennsylvaniaSchool of Biomedical Engineering, Science and Health Systems, Drexel UniversityDepartment of Orthopaedic Surgery, Perelman School of Medicine, University of Pennsylvania||Translational Musculoskeletal Research Center, Corporal Michael J. Crescenz VA Medical CenterDepartment of Orthopaedic Surgery, Perelman School of Medicine, University of PennsylvaniaDepartment of Orthopaedic Surgery, Perelman School of Medicine, University of Pennsylvania||Department of Orthopedics, Sun Yat-Sen Memorial HospitalDepartment of Orthopaedic Surgery, Perelman School of Medicine, University of Pennsylvania||Translational Musculoskeletal Research Center, Corporal Michael J. Crescenz VA Medical CenterDepartment of Orthopaedic Surgery, Perelman School of Medicine, University of Pennsylvania||Department of Orthopaedics, Union Hospital, Tongji Medical College, Huazhong University of Science and TechnologyDepartment of Orthopaedic Surgery, Perelman School of Medicine, University of Pennsylvania||Department of Orthopaedics, Union Hospital, Tongji Medical College, Huazhong University of Science and TechnologyDepartment of Orthopaedic Surgery, Perelman School of Medicine, University of Pennsylvania
基础医学生物科学研究方法、生物科学研究技术生理学
Gli1mesenchymal progenitorlineage tracingmeniscus injuryosteoarthritis
Liu X. Sherry,Zhang Yejia,Koyama Eiki,Long Fanxin,Qin Ling,Gui Tao,Zhong Leilei,Ahn Jaimo,Han Lin,Mauck Robert L,Zgonis Miltiadis,Sun Hao,Heo Su-Jin,Wei Yulong,Yu Wei,Yao Lutian.Identification of Gli1 as a progenitor cell marker for meniscus development and injury repair[EB/OL].(2025-03-28)[2025-05-21].https://www.biorxiv.org/content/10.1101/2020.11.27.401463.点此复制
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