高频重复经颅磁刺激对脑梗死后学习记忆功能及pCREB、bcl-2、bax表达的影响

Objective: To investigate the effects of high-frequency repetitive transcranial magnetic stimulation (rTMS) on learning and memory ability and its mechanism in rats with cerebral infarction Method: Reperfusion model with middle cerebral artery occlusion was established. Repetitive transcranial magnetic stimulation of 20Hz was given to successful models for 7 days. Learning and memory changes of the rats were observed with the Morris water maze. Expression changes of pCREB, bcl-2 and bax were investigated between model control group and rTMS group or blocker injecting group and normal saline injecting group.Result: ①The escape latency in model control group delayed obviously as compared with that in blank control group(p=0.001). The escape latency in rTMS group decreased significantly as compared with that in model control group (p = 0. 017). ②The pCREB and bcl-2 expression increased in the rTMS group as compared with that in the model group (P <0.01) . The bax expression decreased in the rTMS group compared with that in the model group (P <0.01). The ratio of bcl-2 and bax (bcl-2/bax) in the rTMS group increased compared with that in the model group (P <0.01). The pCREB and bcl-2 expression in the blocker injecting group decreased as compared with that in the normal saline injecting group(P <0.01). The bax expression increased in the blocker injecting group compared to that in the normal saline injecting group (P <0.01). The ratio of bcl-2 and bax (bcl-2/bax) in the blocker injecting group fell compared with that in the normal saline injecting group (P <0.05). Conclusion: Repetitive transcranial magnetic stimulation of 20Hz could promote hippocampus neuronal survival after cerebral ischemic and inhibit apoptosis, which was probably realized by its affecting on the pathway of PKA-CREB.