从JNK- caspase-3信号通路研究苦碟子注射液对大鼠急性脑缺血再灌的影响

im：To discuss the possible mechanism of Ku Diezi injection on JNK- caspase-3 signal pathway in the treatment of acute cerebral ischemia. Methods: Animal model of focal cerebral ischemia and reperfusion is the thread occlusion technique induced middle cerebral artery occlusion in rats. Rats were randomly divided into seven groups: sham group, 6h, 24h, 72h of reperfusion respectively after 1.5h of ischemia by transient occlusion of middle cerebral artery, and Ku Diezi injection treatment groups . Detecting the protein expression of JNK/p-JNK and caspase-3 by Western blot. Results：Compared with the sham group, p-JNK and caspase-3 protein expression are peaked in R24h of model group, corresponding time points Ku Diezi injection group reduce the p-JNK and caspase-3 protein expression, relative with statistical significance (P <0.01). Conclusion：Ku Diezi injection probably blocking the JNK signaling pathway, inhibiting caspase-3 protein expression and decreas apoptosis, thereby reducing cerebral ischemia-reperfusion injury.