柑橘β-隐黄质的体外抗氧化活性及其对血管平滑肌细胞增殖的调控研究

he aims of this research were to explore the activities of β-Cryptoxanthin (β-Cry) in vitro by free radical scavenging ability assays, and anti-proliferation effect observation on vascular smooth muscle cell (VSMC). The results showed that β-Cry had highly scavenging abilities of DPPH radical and ABTS radical, and IC50 values were 9.52μg/ml and 8.26μg/ml, respectively, which were better than Trolox. MTT assay implied that β-Cry could inhibit the VSMC proliferation induced by PDGF BB in a dose effect. Cell cycle distribution of VSMC measured by flow cytomety indicated that the proportion of VSMC treated by PDGF BB of G1/G0 phase cells decreased by induction of PDGF BB, and the S phase cells increased in comparison with control group （p<0.05）, which would be in consistent with the cell cycle related protein expression detected by Western blot, as Cyclin E and Cyclin D1 were up regulated, however, p21 was inhibited. In β-Cry+ PDGF BB treated group, β-Cry regulated proportion of G1/G0 phase cells and S phase cells, and the expression of Cyclin E, Cyclin D1 and p21, respectively. In conclusion, β-Cry may inhibit the PDGF BB induced VSMC proliferation via down regulating the expression of cyclin E and cyclin D1 induced by PDGF BB, and up regulating p21 expression, respectively, and this would provide fundamental results for the evaluation of citrus carotenoids bioactivities.