基于单细胞转录组学数据的细胞通讯研究方法与应用

ell-cell communication (CCC) is a basic biological process that regulates the growth and development of organisms, maintains the homeostasis of internal environment and regulates the immune response. When CCC process cannot be carried out normally, it will affect the occurrence and development of diseases. Previous CCC analysis methods were very demanding and could only study CCC of a few molecules in a specific cell type. With the development of single cell transcriptome sequencing (scRNA-seq), a variety of methods for CCC study based on scRNA-seq transcriptome data have been proposed, which makes it possible to construct a more comprehensive map of cell-cell communication. In this paper, we reviewed the methods of CCC inference based on scRNA-seq data in recent years, summarized the current methods of evaluating CCC inference methods and inference results, and finally prospected their development prospects.