罗丹明123在三种乳腺癌细胞中的分布和外排研究

Objective:To observe the distribution and efflux of rhodamine123(Rh123) in human breast cancer sensitive cells（MCF-7/S）and resistant cells（MCF-7/ADR、MCF-7/TAM）,and to preliminary distinguish the mechanism between breast cancer cells resistant to adriamycin and to tamoxifen.Methods:Rh123 was respectively cultured with three breast cancer cells for 20minutes,and Rh123 was then washed away,to observe the distribution and changes of fluorescence intensity in cells at different time points by using laser scanning confocal microscope.Results:Rh123 was mainly distributed in the nucleus in the MCF-7/ADR cells lumpishly,and evenly distributed mainly around the nucleus membrane in the MCF-7/S and MCF-7/TAM cells.And the efflux rate of the former was higher than the latter two.Conclusion:Accelerated drug efflux is the main mechanism in breast cancer cells resistant to adriamycin, and is not the mechanism in breast cancer cells resistant to tamoxifen.