调肝益气通络方促进骨髓间充质干细胞体外增殖的研究

Objective: by observing to tune liver Yiqitongluo side rat mesenchymal stem cell proliferation, to evaluate the proliferation ability of the party. Methods: intraperitoneal injection of CCl4-olive oil the suspension preparation Wistar rats (35) model of hepatic fibrosis; 30 were given tone the liver Qi Tong Luo Fang (low, medium and high dose) month preparation containing serum; 5 Isolation and culture of MSC cells; MSC drug-containing serum-induced liver cell differentiation for 14 days; morphology was observed in the detection and cell proliferation was evaluated by MTT，Detected by Western blot induce the expression of alpha-fetoprotein 7 days and 14 days.Results: The concentration of serum containing added 1 --14days, the number of cells than conventional culture group increased, and proportional to the same dose, the greater the concentration, the more the number of cells, the number of cells over time and increasing，14 days after the induction of high-dose group was strongly positive expression of alpha-fetoprotein. Conclusion: Tiaogan Qi Tong Luo side has a significant role in promoting the proliferation of rat bone marrow mesenchymal quality hepatocytes, And it may contribute to the secretion of alpha-fetoprotein.