检测鼠粪中隐孢子虫卵囊方法的研究

Objective To serve the clinical diagnosis better, Comparative studying the effect of different methods on detecting C.parvum oocysts in mice and cow feces. Methods Feces with grads concentration of oocysts per 10 gram were prepared, 27 mice feces and 33 cow feces were collected, and detected by the 3 methods respectively, modified acid-fast staining(MAFS), immunofluorescence using monoclonal antibody(McAb) immunolabeling by FITC against C.parvum and polymerase chain reaction(PCR). Results The least dosages of oocysts detected using forer 3 metheds in 10 g feces and the least time spended on 3 methods at a time were 2.0×104, 2.0×104, 2.0×102 and 35 min、60min、3h respectively. Masculine ratio in mice and cow feces detected were 65.0 %、70.0 %、85.0 % and 21.21 %、18.18 %、27.27 % respectively, no difference was found among the 3 methods. Conclusion The modified acid-fast staining is operated simply and quickly, which is suited for detecting quantities of samples and spends less with lower condition。Its sensitivity is the same as the method using IFA- McAb, which is suited for identifying the Cryptosporidium oocyst type in human and animals for the use of the immunofluorescence microscope. It is the highest for PCR in the sensitivity of detecting C.parvum oocyst, but PCR apparatus must be used in identifying the oocyst type.