乙醇对CHSE-1细胞中细胞色素P450 2E1的诱导研究

he dose- and time-dependent induction of P450 2E1 by ethanol in CHSE-1cell was investigated in the present study. 48h after inoculation, cells were induced with ethanol of different concentrations for 24h, and then aniline was added, incubated at 28℃ for 30 min. CYP2E1 activity was measured using the product,4-aminophenol as an index. The optimal concentration of aniline was 10～16mM, and basal activity of cells grew into confluence for aniline hydroxylase was 0.152±0.095 nmol•min-1•mg-1TP. The dose-dependent effect was fited by Gentox module, which generated a curve that at first rose and then fell down, indicating that it was firstly induced and inhibited afterward by ethanol. The value of P0, nH, k and EC50 was 0.13967, 2.4575, 0.0244 and 34.9377, respectively. It was also demonstrated that the activity of CYP2E1 induced by ethanol increased with time, the highest value was up to 0.4456±0.0916 nmol•min-1•mg-1TP after 24h which was a typical enzyme induction example. Through this study, the model of CYP2E1 induction by ethanol in CHSE-1 was established，and the parameter of it was analysed.