利用Baclight染色法测定活性污泥中的活菌水平

he BacLight staining method, the reliability of which had been validated by previous experiments, was used to measure the live bacteria ratio in the activated sludge. The density of green fluorescence (Fg) was measured by fluorescence spectroscopy after the samples were stained by SYTO®9 and PI. The Fg indicated the level of live bacteria. When the sludge concentration was lower than 100mg/L, the dose of staining material was 25μl/ml sample. Diluting activated sludge until the concentration was under 50mg/L, the affection of inert floc could be ignored. The sample treated by dispersing by ultrasonic (the duration was 2 min and the power was 40W) could improve the repeatability of the method.