二型糖尿病伴或不伴增生性糖尿病视网膜病变患者循环内皮祖细胞异常状态的研究

Purpose: To investigate the number and function of circulating endothelial progenitor cells (EPC) in patients with type 2 diabetes mellitus (T2DM) with or without proliferative diabetic retinopathy (PDR). Methods: Circulating EPCs were isolated from 30 T2DM patients with PDR (60 eyes), 30 T2DM patients without PDR (60 eyes), as well as age matched control subjects (n=20). EPCs isolated from peripheral blood were quantified using flow cytometric analysis to count the number of CD34+, CD133+and VEGF R2+ cells. Following isolation, the EPCs were cultured in vitro to evaluate proliferation, adhesion and migration. Results: The number of circulating EPCs was significantly decreased in T2DM patients with and without PDR when compared with the healthy control individuals. In addition, the number of EPCs in the cultured samples from T2DM patients with and without PDR was lower compared with the control individuals. Furthermore, impaired proliferation, adhesion and migration were observed in the cultured EPCs from T2DM patients with and without PDR. Notably, increased numbers and aggravated dysfunction of EPCs were detected in T2DM patients with PDR compared with T2DM patients without PDR. In addition, a positive correlation was identified between the EPC number and PDR duration in T2DM patients with PDR.Conclusion: Reduced number and impaired function of circulating EPCs were observed in T2DM patients with or without PDR. In conclusion, EPC levels and aggravated EPC dysfunction in patients with PDR compared with patients without PDR may contribute to the pathogenesis of PDR in T2DM patients. EPC levels may be used as indicators in order to monitor PDR.